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|Title||Immune-detection of Entamoeba histolytica in symptomatic and asymptomatic infection|
High prevalence of intestinal amoebiasis is commonly reported by microscopy in Gaza.To determine the misdiagnosis of intestinal amoebiasis associated with microscopic examination of faces, two tests were applied: 1-a non-specific antigen Entamoeba ( ELISA to differentiate Entamoeba histolytica/Entamoeba dispar complex from other non-pathogenic intestinal amoebae.2- An enzyme-linked immunosorbent assay ( ELISA for detecting antibody against E. histolytica, 53 stool and serum samples were used in this study: 32 ( 60.38% samples were symptomatic ( diarrheal patients and 21 ( 39.62% were asymptomatic. Data from these two immunological diagnostic tests were compared with those obtained by microscopic examination. A routine microscopic examination detected 7 ( 13.25 prevalence of Giardia lamblia, 21 ( 39.6% prevalence of Entamoeba spp. 13 ( 40.6%; X2=0.578; P-value= 0.749 in symptomatic stool samples and 8 ( 38.1% in asymptomatic stool samples. ELISA antigen detected only 2 ( 6.2%; X2=1.364; P-value= 0.243 prevalence of E. histolytica/ dispar complex in symptomatic samples and none in asymptomatic samples. The two positive symptomatic samples by ELISA/Ag were also positive by microscopy, and no negative subject by microscopy was positive by ELISA. ELISA antibody detected 4 ( 7.5%; X2=0.195; P-value= 0.659 prevalence of E. histolytica in all subjects: 2 ( 6.2% in symptomatic samples which were positive by both microscopy and ELISA antigen and 2 ( 9.5% in asymptomatic samples, that were negative by both microscopy and ELISA antigen. The ratio of E. histolytica/ dispar was very low ( 3.7% suggesting that the vast majority of Entamoeba infections in this area were nonpathogenic. The microscopic examination is less sensitive than both ELISA/Ag and ELISA/IgG tests.
|Series||Volume: 1, Number: 5|
|Publisher||J Am Sci|
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