Isolation and molecular characterization of cry gene for Bacillus thuringiensis isolated from soil of gaza strip

Abstract

Bacillus thuringiensis, insecticide, polymerase chain reactions (PCR), crystalline protein, Cry gene, Gaza strip. Bacillus thuringiensis (Bt) is a rod-shaped, gram-positive, facultative anaerobic, and spore-forming bacterium (Konecka et al., 2007). During sporulation, it produces insecticidal proteins, which are deposited within the sporangium as crystalline aggregates (Crickomre et al., 1998;