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http://hdl.handle.net/20.500.12358/25859
TitlePeroxide optrode based on micellar-mediated chemiluminescence reaction of luminol
Untitled
Abstract

Cetyltrimethylammonium bromide (CTAB) was used to overcome the pH mismatch of the luminol (5-amino-2,3-dihydrophthalazine-1,4-dione ) chemiluminescence reaction when coupled to the glucose/glucose oxidase reaction at neutral pH. The results demonstrate the feasibility of conducting both reactions simultaneously and efficiently at pH 7.5–8.5. The incorporation of the CTAB micellar system in the coupled luminol/enzymatic reaction allows quantification of glucose in the 3 × 10−7−3 × 10−4 M range. The relative standard deviation (RSD) for 5 replicates of 5 × 10−5 M glucose was 3.8%. Also, hydrogen peroxide was quantified in the 1.2 × 10−4−2.4 × 10−8 M range with RSD 2.6%. The micellar-mediated luminol reaction was applied successfully to the determination of glucose in blood serum. Excellent agreement with reported results by standard assays was obtained.

Authors
Abdel-Latif, Monzir S.
Guilbault, George G
TypeJournal Article
Date1989
Published inAnalytica Chimica Acta
SeriesVolume: 221
PublisherElsevier
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The institutional repository of the Islamic University of Gaza was established as part of the ROMOR project that has been co-funded with support from the European Commission under the ERASMUS + European programme. This publication reflects the views only of the author, and the Commission cannot be held responsible for any use which may be made of the information contained therein.

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The institutional repository of the Islamic University of Gaza was established as part of the ROMOR project that has been co-funded with support from the European Commission under the ERASMUS + European programme. This publication reflects the views only of the author, and the Commission cannot be held responsible for any use which may be made of the information contained therein.

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