Peroxide optrode based on micellar-mediated chemiluminescence reaction of luminol

Abstract

Cetyltrimethylammonium bromide (CTAB) was used to overcome the pH mismatch of the luminol (5-amino-2,3-dihydrophthalazine-1,4-dione ) chemiluminescence reaction when coupled to the glucose/glucose oxidase reaction at neutral pH. The results demonstrate the feasibility of conducting both reactions simultaneously and efficiently at pH 7.5–8.5. The incorporation of the CTAB micellar system in the coupled luminol/enzymatic reaction allows quantification of glucose in the 3 × 10−7−3 × 10−4 M range. The relative standard deviation (RSD) for 5 replicates of 5 × 10−5 M glucose was 3.8%. Also, hydrogen peroxide was quantified in the 1.2 × 10−4−2.4 × 10−8 M range with RSD 2.6%. The micellar-mediated luminol reaction was applied successfully to the determination of glucose in blood serum. Excellent agreement with reported results by standard assays was obtained.