Common Interleukin-18 Gene Polymorphisms in Women with Recurrent Spontaneous Abortion in Gaza Strip-Palestine

Abstract

Accepted: 12 Dec 2014 Background: This work was carried out in order to investigate the association between Interleukin-18 (IL-18) gene SNPs (+ 105A> C," rs549908";-137 G> C," rs187238";-607 C> A," rs1946518"; and-656 G> T," rs1946519") and RSA among Palestinian women residing in Gaza Strip. Methods: In this case-control study, samples from 200 women (100 suffering from RSA and 100 control) were examined. All participants were genotyped for IL-18 SNPs:-137 G> C,-607 C> A,+ 105A> C, and-656 G> T. Allele specific polymerase chain reaction (AS-PCR) was used to detect IL-18-137 G> C and-607 C> A SNPs whereas, restriction fragment length polymorphism (RFLP-PCR) method was used for genotyping IL-18+ 105A> C and-656 G> T polymorphisms. Results: The results revealed that there is no significant association between the allele/genotype frequencies of the investigated IL-18 SNPs and RSA in the study population. Though not significant, the two groups showed remarkable differences in terms of allele/genotype frequencies of SNP-137 G> C. Conclusion: Among the examined IL-18 SNPs the promoter-137 G> C may be important in RSA in the investigated population.