Ablation of neuronal ceramide synthase 1 in mice decreases ganglioside levels and expression of myelin-associated glycoprotein in oligodendrocytes

Abstract

Background: Ceramide synthase 1 catalyzes the synthesis of C18 ceramide and is mainly expressed in neurons of the brain. Results: Ablation of Ceramide Synthase 1 decreases ganglioside levels and expression of oligodendrocytic Myelin Associated Glycoprotein in motor impaired mice. Conclusion: CerS1-derived C18-gangliosides are essential for cerebellar development and neurodevelopmentally-regulated behavior in mice. Significance: Neuronal gangliosides regulate expression of Myelin associated Glycoprotein in oligodendrocytes. Abstract Ceramide synthase 1 (CerS1) catalyzes the synthesis of C18 ceramide and is mainly expressed in the brain. Custom-made antibodies to a peptide from the C-terminal region of the mouse CerS1 protein yielded specific immuno-signals in neurons and no other cell types of wild type brain, but did not detect the CerS1 protein in CerS1 deficient mouse brains. To elucidate …