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http://hdl.handle.net/20.500.12358/24113
TitleLeptin Adversely Affects The Transition of Histone-to-Protamine during Spermatogenesis in Sprague-Dawley Rats
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Abstract

The aim of this study was to examine the effect of leptin on the histone to protamine ratio in adult rat sperm. Twelve-week old Sprague-Dawley rats were randomized into control and leptin-treated groups with 6 rats per group.  Leptin-treated group received intra-peritoneal injections (i.p.) of leptin daily for 42 days (60 µg/kg body weight).  Control rats received 0.1 ml of 0.9 % saline i.p. for 42 days.  Upon completion of the treatment sperm count, morphology and histone-to-protamine ratio measurements were performed.  Gene expressions of HAT, HDAC1, HDAC2, H2B, H2A, H1, PRM1, PRM2, TNP1 and TNP2 were analyzed using microarray analyses.  Data were analyzed using ANOVA.   The results showed that, sperm count was significantly lower among leptin-treated rats compared to control one, whereas the fraction of sperm with abnormal morphology, the histone-to-protamine ratio and the expressions of HAT, HDAC1, HDAC2, H2B, H2A, H1, PRM1 were significantly higher in leptin treated rats.  As a conclusion, exogenous leptin administration adversely affects sperm count and morphology, which might be related to alteration in the histone-to-protamine transition during spermatogenesis.

Authors
Alambhouh, Fayez A.
Singh, Harbindar J.
TypeJournal Article
Date2017
LanguageEnglish
Subjects
histone
leptin
gene expression
protamine
sperm count
Published inIUG Journal of Natural Studies
SeriesVolume: 25, Number: 3
Publisherالجامعة الإسلامية - غزة
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  • Staff Publications- Faculty of Science [1030]
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The institutional repository of the Islamic University of Gaza was established as part of the ROMOR project that has been co-funded with support from the European Commission under the ERASMUS + European programme. This publication reflects the views only of the author, and the Commission cannot be held responsible for any use which may be made of the information contained therein.

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The institutional repository of the Islamic University of Gaza was established as part of the ROMOR project that has been co-funded with support from the European Commission under the ERASMUS + European programme. This publication reflects the views only of the author, and the Commission cannot be held responsible for any use which may be made of the information contained therein.

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