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http://hdl.handle.net/20.500.12358/24092
TitleMelatonin Prevents the Adverse Effects of Leptin on Histone-to-Protamine Transition during Spermatogenesis in Rats
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Abstract

The aim of this study was to examine the effect of melatonin on leptin induced changes in transition of histone to protamine in adult rats during spermatogenesis. Twelve-week old Sprague-Dawley rats were randomized into control, leptin, leptin-melatonin-10, leptin-melatonin-20 and melatonin-10 treated groups with 6 rats per group. Leptin-treated groups received intra-peritoneal injections (i.p.) of leptin daily for 42 days (60 µg/kg body weight). Rats in the leptin and melatonin treated groups were given either 10 or 20 mg of melatonin/day/kg body weight in drinking water. Melatonin-10 treated group received only 10 mg of melatonin/day/kg body weight in drinking water for 42 days. Control rats received 0.1 ml of 0.9 % saline i.p. for 42 days. Upon completion of the treatment, sperm count, morphology and histone-to-protamine ratio estimation was performed. The expression of HAT, HDAC1, HDAC2, H2B, H2A, H1, PRM1, PRM2, TNP1 and TNP2 genes were analyzed using microarray analyses. Data were analyzed using ANOVA. Sperm count was significantly lower whereas the fraction of sperm with abnormal morphology, the ratio of histone-to-protamine transition and the level of HAT, HDAC1, HDAC2, H2B, H2A, H1, PRM1 were significantly higher in leptin treated rats. No significant differences were evident in these between controls and melatonin treated rats. It appears that exogenous leptin administration adversely affects histone-to-protamine transition, upregulates the genes involved in this replacement and these effects are prevented by concurrent administration of melatonin.

Authors
Alambhouh, Fayez A.
TypeJournal Article
Date2017
LanguageEnglish
Subjects
melatonin
sperm
leptin
protamine
histone
Published inIUG Journal of Natural Studies: (Special Issue) The Sixth International Conference on Science & Development � 14-15 march 2017
SeriesVolume: 25, Number: 2
Publisherالجامعة الإسلامية - غزة
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  • Staff Publications- Faculty of Science [1066]
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The institutional repository of the Islamic University of Gaza was established as part of the ROMOR project that has been co-funded with support from the European Commission under the ERASMUS + European programme. This publication reflects the views only of the author, and the Commission cannot be held responsible for any use which may be made of the information contained therein.

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The institutional repository of the Islamic University of Gaza was established as part of the ROMOR project that has been co-funded with support from the European Commission under the ERASMUS + European programme. This publication reflects the views only of the author, and the Commission cannot be held responsible for any use which may be made of the information contained therein.

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