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http://hdl.handle.net/20.500.12358/24026
TitleThermodynamic Properties of Hydrogen Peroxide Binding to Bovine Liver Catalase
Untitled
Abstract

Catalase [CAT: EC 1.11.16] was purified from bovine liver using cellulose affinity chromatography and some enzyme characteristics were investigated. The interaction of H2O2 with catalase was investigated by fluorescence spectroscopy, and the change in intrinsic fluorescence intensity at 435 nm was used to estimate the association constant of the interaction. The binding process resulted in a change in the intrinsic fluorescence. The emission spectra were analyzed according to a model described by Chipman et al., to obtain the association constant (ka ) values in the temperature range of 25-50 ?C. The results showed that the association constants were temperature dependent. The value of Ka at 25°C was 2.4 × 105 M –1. Moreover, the thermodynamic parameters for the interaction H2O2/BLC were obtained from Van't Hoff plot. The results indicated that the interaction was enthalpically driven accompanied by negative entropic contribution in the studied temperature range. The association constant values were determined from Van't Hoff plots. Key words: Bovine liver catalase, fluorescence emission, association constants, thermodynamic parameters, Chipman model.

Authors
Abu-Jadallah, SalahEdeen Y.
Yasseen, Zeyad J.
TypeJournal Article
Date2009
LanguageEnglish
Subjects
fluorescence emission
association constants
bovine liver catalase
thermodynamic parameters
chipman model
Published inIUG Journal for Natural and Engineering Studies
SeriesVolume: 17, Number: 1
Publisherالجامعة الإسلامية - غزة
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  • Staff Publications- Faculty of Science [1066]
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The institutional repository of the Islamic University of Gaza was established as part of the ROMOR project that has been co-funded with support from the European Commission under the ERASMUS + European programme. This publication reflects the views only of the author, and the Commission cannot be held responsible for any use which may be made of the information contained therein.

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The institutional repository of the Islamic University of Gaza was established as part of the ROMOR project that has been co-funded with support from the European Commission under the ERASMUS + European programme. This publication reflects the views only of the author, and the Commission cannot be held responsible for any use which may be made of the information contained therein.

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