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|Title||Effect of Origanum syriacum, Hibiscus sabdariffa, Thymus vulgaris and Pelargonium graveolens Extracts on Human Leukemia THP-1 Cells in Vitro|
Introduction: Leukemia is an aggressive disease that if untreated it leads to death. Because the conventional leukemia treatment protocols are not effective in all cases and result in serious side effects, discovering new safer and more effective anticancer agents from natural materials is the aim of many medical researches nowadays. Within this perspective, the present study was constructed to assess the possible anticancer effects of Origanum syriacum, Hibiscus sabdariffa, Thymus vulgaris, and Pelargonium graveolens, extracts on human leukemic THP-1 cells and to evaluate the effect of these extracts toward normal human peripheral blood mononuclear cells (PBMCs). Methodology: The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay was used to assess the antiproliferative effects while cytotoxicity was evaluated using the lactate dehydrogenase (LDH) assay. Ethanol extracts of the four plants were prepared at various concentrations then were investigated against both THP-1 cells and PBMCs by MTT assay. After that, three of the extracts were tested with LDH assay against THP-1 cells. Results: All extracts, except P. graveolens extract, exhibited a concentration dependent antiproliferative effect against THP-1 leukemic cells with IC50 values of 15.47 mg/mL for H. sabdariffa, 2.126 mg/mL for O. syriacum, and 0.1569 mg/mL for T. vulgaris. Also they showed inhibition against PBMCs with IC50 values of 3.514 mg/mL for H. sabdariffa, 0.4247 mg/mL for O. syriacum, and 0.3345 mg/mL for T. vulgaris. Only O. syriacum extract exerted a cytotoxic effect against leukemic cells, as evaluated by the LDH assay, with LC50 value of 9.646 mg/mL while T. vulgaris and P. graveolens extracts did not show any cytotoxicity. Conclusion: It can be concluded that only T. vulgaris extract, which showed a relative selective inhibition toward leukemic cells, may be useful in developing successful treating agents for leukemia.
|Publisher||الجامعة الإسلامية - غزة|
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