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|Title||Significance of Some Trace Elements in Seminal Plasma of Infertile Men in Gaza Strip|
|Title in Arabic||اهمية بعض العناصر النادرة في بلازما السائل المنوي لمرضى قلة الخصوبة في قطاع غزة|
Infertility is considered a worldwide problem which is thought to be caused by multiple factors. The role of trace elements in male fertility has been considered. It has been indicated that certain levels of zinc, copper, iron, and magnesium are necessary for both spermatogenesis and steroidogenesis. The suggested roles of these elements in male infertility lead to the introduction of these elements as supplementary elements for improvement of male fertility. This study is aimed to compare the levels of zinc, copper, iron and magnesium in the seminal plasma of infertile group and control group, to find out relationships in male infertile group between these elements and both seminal characteristics, serum reproductive male hormones (Luteinizing hormone, Follicle stimulating hormone, and testosterone), and also to understand the biological significance of such elements in male infertility. Seventy two infertile male subjects, without any treatment who had regular unprotected intercourse for at least 12 months without conception, aged 20-50 years, were selected from the central infertility center Al Shifa hospital, Gaza, Palestine. Seventy two known fertile males selected as control group from general population (their wives had given birth to a child within one year). Semen samples analyzed according to WHO criteria and seminal plasma trace elements were analyzed by atomic absorption spectrophotometry. Serum samples for endocrine studies were measured by (Enzyme Linked Immunosorbent Assay) ELISA, using kits supplied by Diagnostic System Laboratories (DSL),USA. Our results showed that the mean values of zinc, copper and magnesium, were significantly lower in infertile men compared to controls (68.9 mg/L, 300mg/L and 67.1mg/L) versus (122mg/L, 377mg/L and 120 mg/L respectively), while iron mean values were significantly higher in infertile men seminal plasma compared to the controls 412mg/L versus 329 mg/L. Almost all studied seminal parameters (sperm count, forward motility, weak motile and non motile) were significantly lower in infertile group compared to controls (P<0.001, P<0.001, P<0.05, P<0.001, respectively) as well as hormonal parameters (LH and Testosterone) in the infertile group were significantly lower compared to the control group (p<0.05). There was no significance difference between means of FSH levels (p>0.05). Correlation analysis in infertile group showed that while seminal plasma zinc and magnesium levels are directly proportional to sperm count (r=0.376, P=0.001 and r=0.293, P=0.013 respectively), and testosterone (r=0.293, P=0.012 and r=0.324, P=0.003 respectively), it is indirectly proportional to seminal volume (r=-0.251, P=0.034 and r=-0.369 P=0.001 respectively). In contradiction copper did not show any significant relations with any of the seminal or hormonal parameters. Seminal plasma levels of iron were found to show correlations with sperm count and FSH levels. Seminal plasma iron levels were directly proportional to sperm count (r=0.290, P=0.014) and indirectly proportional to FSH levels(r=-0.349, P=0.003). Trace elements inter-correlations showed that both magnesium and zinc levels are directly proportional to each other (r=0.744, P=0.0001). The results obtained in our study indicate that both zinc and magnesium are important for both spermatogenesis and steroidogenesis. The results also indicate that both zinc and magnesium are important extracellularly (in the seminal plasma) while iron is important intracellulary (in sertoli cells). While magnesium and zinc are important for steroidogenesis, increased serum plasma levels of FSH increases the uptake of iron in sertoli cells. To the best of our knowledge, this is the first study in Gaza Strip investigating the significance of trace elements in seminal plasma and serum endocrine parameters in infertile men.
|Publisher||the islamic university|
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