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|Title||Improving the Diagnosis of Dermatophytes in Gaza Strip by using Nested PCR|
Dermatophytes are a very related to keratinophilic fungi that can invade keratinized humans and animal tissues such as skin, hair and nails causing dermatophytosis. They are the important cause of superficial fungal infection. Conventional methods like potassium hydroxide (KOH) microscopy and fungal culture lacks the ability to make an early and specific diagnosis. In this study it is taken into consideration to evaluate nested polymerase chain reaction (NPCR) using primers targeting dermatophyte specific sequence of chitin synthase 1 (CHS1) gene and compared with conventional method potassium hydroxide (KOH) microscopy test in Remal Clinic inGazacity. A total of ninety nine patients were clinically suspected with dermatophytosis including 16 skin specimens 16 nail specimens and 67 hair specimens. For each specimens KOH, PCR and NPCR tests were carried out. Having compared the output results of NPCR sequencing with the wild-type gene which is obtained from the National Center for Biotechnology Information (NCBI) gene bank. The comparison indicates that the product of NPCR is CHS1 gene according to (NCBI) gene bank. Additionally, it is considered to compare the results of NPCR with KOH for dermatophytes which gives that 41.4% are positive indication based on KOH and 18.18% is positive indication according to NPCR. After carrying out the statistical analysis using SPSS for both tests results obtained from NPCR and KOH, it is found that 30% of the total sample has to be included for treatment based on KOH test, although this percent of the sample doesn’t need to undergo treatment according to NPCR test. It is also shown that 6% of the sample are excluded for treatment in KOH test, in spite the NPCR indicated that this percent must be included in the treatment. The prominent controversy between the test results (KOH and NPCR) was found particularly in the nails diagnosis.
|Publisher||الجامعة الإسلامية - غزة|
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