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|Title||Single Nucleotide Polymorphism (E23K) of KCNJ11 Gene and other Risk Factors Associated with Type 2 Diabetes Mellitus Patients in Gaza City- Palestine|
|Title in Arabic||الطفرة احادية النيكلوتيدة (e23k) للجين kcnj11 وعوامل خطر اخرى مرتبطة بمرضى السكري من النوع الثاني بمدينة غزة-فلسطين|
Type 2 diabetes mellitus (T2DM) is a multifactorial disease in which environmental triggers interact with genetic variants in the predisposition to the disease. KATP channels regulate insulin secretion by coupling the metabolic state of the cell to membrane potential. (KATP) channels in pancreatic β-cells comprise a pore-forming, K+ inward rectifier (Kir6.2) encoded by the KCNJ11 gene. A common single nucleotide polymorphism E23K of the KCNJ11 gene has been associated with T2DM in different population. The role of this polymorphism was not previously tackled in Palestine. Therefore, the aim of this study is to assess the (E23K) SNP in KCNJ11 gene as a possible contributor to T2DM among Gaza City (GC) patients. Also to consider relation between E23K SNP and other environmental/physical risk factors for T2DM. In order to achieve this goal, two hundred male and female individuals were examined: 100 T2DM patients and 100 control individuals. The glucose level was determined, and DNA was extracted from whole blood samples for all subjects. The groups were genotyped of SNP (E23K) by PCR/RFLP technique using the BanII restriction enzyme. A questionnaire was completed to evaluate the role of physical and environmental risk factors for T2DM. The results of the study showed that there was a strong statistically significant relation between the distribution of the E23K polymorphism and T2DM (P=0.000). Fourty-three percent of cases were E/K compared to 29% of controls; and 15% of cases were K/K compared to 3% of controls. Obesity, persistent stress, absence of physical activity and low level of education are also statically significant related to T2DM in the GC (P=0.000). Moreover, the mean fasting blood sugar level was statistically higher among the cases than the control, and particularly among the cases having any of the upper mentioned genetic and\or environmental risks factors (P= 0.000). The highest percentage of cases with family history of T2DM was among those of E/K (88.4%) compared to (73.3%) of K/K, and (37.8%) in E/E. This distribution was not statistically significant (P=0.191). In a conclusion risk factors that are significantly implicated with T2DM in diabetic patients in the GC include the E23K polymorphism, obesity, stress, absence of physical activity, and low level of education. The inheritance of the K allele predisposes for T2DM , provided that other genetic or/and physical and environmental risk factors to be present.
|Publisher||the islamic university|
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