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|Title||Detection of Methicillin-Resistant Staphylococcus aureus in Nosocomial Infections in Gaza Strip|
Although Staphylococcus aureus is a member of the normal human microbiota (flora), it may cause fatal infections to humans who underwent accidental injury or surgical operation. The bacterium is potent in acquiring antibiotic resistance, and is now a very important causative agent of hospital acquired infections. In Gaza Strip hospitals, as in other parts of the world, methicillin resistant S. aureus (MRSA) is considered one of the most important causative agents of nosocomial infections, so the present study aimed broadly to obtain a snapshot of MRSA prevalence in Gaza Strip, a part of the world not previously surveyed for this type of resistance, as well as the antibiotic resistance pattern of these isolates. A total of 150 clinical isolates of S. aureus were identified from different patients. Disk diffusion tests, CHROMagar MRSA medium, and the PCR assay technique were performed for each of the 150 isolates to identify MRSA strains. The prevalence of methicillin resistance among S. aureus isolates was 22% (33 isolates), which can be considered a low percentage compared to that in neighboring countries. Detection of MRSA in hospital laboratories highlights the epidemiology of MRSA, and decreases the creation of more resistant strains that may result from random empirical treatment through the use of unnecessary wide spectrum antibiotics. The results of this study show that using methicillin conventional disk diffusion test for detection of MRSA in hospital laboratories is highly reliable and it can reach the same value of specificity and sensitivity of the PCR assay. Overcrowding of the patients and long hospitalization are the major causes of MRSA distribution among patients, as shown from the investigations conducted in the European hospital. It is recommended to rapidly detect MRSA among hospitalized patients and isolate any patient who is infected with MRSA, as well as making routine MRSA detection in hospital wards to identify any new emergence of MRSA.
|Publisher||the islamic university|
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