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|Title||Common CYP2C19 Alleles in Gaza Strip Population|
CYP is a heme-containing enzyme present in liver, widely distributed from bacteria to mammals, and comprises a superfamily of metabolic enzymes that play an important role in the metabolism of endogenous substrates, environmental toxins, carcinogenic substances and a variety of pharmaceuticals. CYP2C19 is one of the most important members of these enzymes. Genetic polymorphism of CYP2C19 can lead to sever toxicity or therapeutic failure of medications as well as to a possible increase in an individual’s susceptibility to certain types of chemically induced cancers and other diseases. The aims of the present study was to determine the frequencies of CYP2C19*1, CYP2C19*2 and CYP2C19*3 alleles and the associated CYP2C19 genotypes in Gaza Strip population. The study was conducted on 200 unrelated healthy human volunteers. DNA was extracted from blood samples and analyzed by the PCR-RFLP method. The PCR product was digested with restriction enzymes (SmaI and BamHl) and then separated electrophoretically using 3% agarose gel. The frequencies of the CYP2C19*1, *2 and *3 alleles were 91.3, 5.8 and 3.0%, respectively. The distributions of CYP2C19*1/*1,*1/*2, *1/*3, *2/*2, *2/*3 and *3/*3 genotypes were 86.5, 6.5, 3.0, 1.5, 2.0, and 0.5 %, respectively. The distribution of CYP2C19*2 in the Gaza Strip population is lower than that in Caucasians, Africans and the Asian populations. The CYP2C19*1 however, is significantly higher in Gaza Strip when compared with other populations. The CYP2C19*3 allele, which was not reported in the Caucasian populations has been identified in 3.0% of the Gaza Strip population.
|Publisher||the islamic university|
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