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|Title||Evaluation of Antibacterial And Synergistic/Anatagonistic Effect of some medicinal Plants Extracted By Microwave And Conventional Methods|
|Title in Arabic||تقييم الفعالية الضد بكتييرية والتظافرية/المثبطة لنباتات طبية تم استخلاصها بطريقة تقليدية وبالمايكروويف|
Abstract The present study was designed for evaluation of a modern method of extraction using Microwave (MW) as an apparatus of extraction process and comparing this method with other conventional method as a soxhlet which used in this study. The bioactive compounds were extracted from the dried plants (Ficus sycomorus leaves, Lawsonia inermis leaves and Glycerrhiza glabra linn) were successively extracted with ethanol 80% and distilled water. Nine samples of each plant for both solvents were irradiated with MW at several power output (180w, 360w and 540w) in several interval times (1, 2 and 3 minuets). The antibacterial activities of extracts and the synergistic effect between plants and antibiotics were evaluated using disk diffusion method against clinical isolated Staphylococcus aureus and Escherichia coli. The result revealed that, the inhibition zone for more than 50% of aquatic and ethanolic samples-d results (extracted in two minutes and MW power 180w) had shown the optimum extract for each plant; as on a consideration sample-d has had shown a maximum magnitude of inhibition zone, though was the lowest time and power. The results of this study showed that ethanolic extracts used against selected microorganisms were showed antimicrobial and synergistic effect with most antibiotics better than aquatic extracts. Where in case of aquatic extracts; G. glabra had the best against E. coli. In case of ethanolic extracts, the best activity was observed with G. glabra against E. coli. Also, synergistic activity of the plant extracts, in case of aquatic extracts; F. sycomorus had the best synergism against S. aureus & E. coli. In case of ethanolic extracts, the best synergism was observed with L. innermis against E. coli, and with F. sycomorus against S. aureus. High levels of antagonism reaction exhibited by all aquatic plant extracts of both methods when combined with antibiotics which showed sensitivity when tested as alone against S. aureus. Also all ethanolic extracts of L. innermis and G. glabra of both methods exhibited antagonism reactions when combined with antibiotics which showed sensitivity when tested as alone against S. aureus. In addition, antagonism reaction occurred against E. coli for antibiotics which were resistant against E. coli when combined with aquatic & ethanolic extracts for both methods of G. glabra which showed sensitivity when tested as alone against E. coli. In addition, a dillution reactions against S. aureus occurred with some antibiotics combined with ethanolic extracts of F. sycomorus & L. innermis. Thereby, our results indicate the possibility of using MW apparatus as an extractor to obtain bioactive compounds from plants and thus used in the treatment of bacterial infections, and some results of this study was encouraging. However, the antagonistic reactions of some extracts with some antibiotics and their use in combination should be further studied for in vitro activities. It is clearly needs to be furthermore evaluated, to identify the effective components, the mode of action and the possible toxic effect in-vivo of these ingredients. Key words. Microwave, soxhlet, antibacterial, synergistic, antagonistic, aquatic extract & ethanolic extract.
|Publisher||الجامعة الإسلامية - غزة|
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