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|Title||Enhancement of Salinity Tolerance in Dianthus caryophyllus L. (omaggio) Using in Vitro Selection Technique and Isolation of Potential Salinty Tolerance Genes from Mesembryanthemum Crystallinum L.|
|Title in Arabic||تحسين قدرة القرنفل على تحمل المياه المالحة باستخدام تقنية الانتخاب المخبري و عزل الجينات المقاومة للملوحة من نبات الغاسول|
Introduction: Salinity is one of the most serious problems affecting the agricultural productivity. Carnation is a glycophyte ornamental plant of major economical value in Gaza Strip where salinity is an ever-increasing problem. Thus the development of new cultivar of carnation with more salt tolerance is considered of high interest to increase the productivity and the plant export. Objective: The objective of this study was to produce new salt tolerance cultivar of Dianthus caryophyllus L. (omaggio) using in vitro selection technique and isolation of potential salinity tolerance genes from Mesembryanthemum crystallinum L. Methodology: Total RNA was isolated from M. crystallinum L. leaves and amplified cloned via reverse transcriptase polymerase chain reaction (RT-PCR) with specific and non- specific primer used under conditions with variable stringency. Nods of carnation were grown directly on media containing 0, 50, 100, 150 and 200 mM NaCl in order to select new cell lines have the ability to withstand high level of salt in in vitro and ex vitro conditions. Results: Two genes were isolated and identified by sequence analysis from M. crystallinum L. as the putative thioredoxin gene, a salt tolerance gene and the serine/threonine-protein kinase EDR1 gene which is usually activated under biotic stress. Carnation tissues were successfully selected and propagated under salinity conditions reaching 100 mM NaCl (EC 10 dS/m) in the Murashige and Skoog (MS) media. However, a final concentration of 200 mM and higher (EC 20 dS/m) was found cytotoxic for all the cells of carnation under the culture conditions. Conclusion: The isolated salt tolerance gene (putative thioredoxin) and stress gene (serine/threonine-protein kinase EDR1) from a wild plant may be useful for later genetic modification of carnation as well as other plants to increase their salinity tolerance. The successful propagation of carnation nods under high concentration of salt in the culture medium represent a promising technique to create cultivars of carnation capable to grow at the salt conditions of Gaza Strip. The sustained propagation of our cultured carnation tissue under high salinity indicates that they may have undergone genetic changes enabling them to adapt to the character of salt tolerance.
|Publisher||الجامعة الإسلامية - غزة|
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