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|Title||Detection of Some Enzymes and Transferrin as Early Diagnostic Markers for Diabetic Nephropathy among Type-2 Diabetic Patients in Gaza|
Kidney Function assessment is important in diabetic patients and early detection of diabetic nephropathy in preclinical stage of disease and will contribute to decreasing morbidity and mortality rates. This study focused on the determination of N-acetyl-ß-D-glucosaminidase (NAG) and gamma glutamyl transferase (GGT) enzymes, transferrin (TRF) and creatinine (CR) in urine of type-2 diabetic subjects and controls of healthy subjects for detection of the relationship between these markers and diabetic nephropathy in type-2 diabetic patients in Gaza. Early morning urine samples were collected from 100 diabetic patients, their age range was from 40-60 years and some of them have hypertension and from 80 control non diabetic subjects with the same age. All the 180 subjects did not suffer from urinary tract infection, liver or renal diseases. In addition, the control subjects did not suffer hypertension, or abnormalities in carbohydrate metabolism. NAG and GGT were measured in urine samples using colorimetric assay kits while TRF was assayed using a turbidimetric immunoassay kit. CR was assayed using the alkaline picrate method. Urinary TRF concentration, NAG and GGT activities showed significant differences between diabetic patients and control. The study showed also that these markers did not have significant difference between hypertensive and non-hypertensive diabetic patients and no significant difference between males and females of patients group. Furthermore, the study showed that 32% have shown elevated NAG excretion but GGT excretion was found elevated only in 15% of cases and TRF was found higher in 29% of patients. One female patient had abnormally elevated levels of TRF, NAG and GGT. No correlations was found between age, body mass index (BMI), smoking, duration of diabetes, systolic or diastolic pressure and these markers. This study indicates that the appearance of the NAG, GGT and TRF in urine may be useful as a non-invasive surrogate test in detecting diabetic nephropathy at an early stage.
|Publisher||the islamic university|
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