Please use this identifier to cite or link to this item:
|Title||Association between Interleukin-18 Polymorphisms and Recurrent Spontaneous Abortion in Gaza Strip-Palestine|
Background: Recurrent spontaneous abortion (RSA), the repetition of three or more consecutive abortions before the 24th week of pregnancy, occurs in approximately 0.5–3% of reproductive-aged women. Immunological factors play an important role in establishing a successful pregnancy. Evidence has accumulated indicating that cytokines play an important role in the maintenance of pregnancy by modulating the immune system. IL-18 is a pro-inflammatory cytokine that plays a central role in inflammation and immune response, and is generally acknowledged as a key defense cytokine against infectious agents. Four polymorphisms in the IL-18 gene at positions -137 G>C, -607 C>A, +105A>C, and -656 G>T, appear to have functional impact as risk factors for RSA. Aim: To investigate the association between IL-18 gene polymorphisms (-137 G>C, "rs187238"; -607 C>A, "rs1946518"; +105A>C, "rs549908"; and -656 G>T, "rs1946519") and RSA among Palestinian women residing in Gaza Strip. Methods: 200 women were examined: 100 women suffering from RSA and 100 control women. The groups were genotyped for IL-18 (-137 G>C, "rs187238"; -607 C>A, "rs1946518"; +105A>C, "rs549908"; and -656 G>T, "rs1946519") polymorphisms. Allele specific polymerase chain reaction (AS-PCR) was used to detect IL-18 -137 G>C, "rs187238" and -607 C>A, "rs1946518" polymorphisms whereas the restriction fragment length polymorphism (RFLP-PCR) method was used for genotyping IL-18 +105A>C, "rs549908"; and -656 G>T, "rs1946519" polymorphisms. Results: There was no significant association between the allele and genotype frequencies of the four single nucleotide polymorphisms (SNPs) in the IL-18 gene and RSA in our population. Conclusion: There was no significant difference detected in the IL-18 gene SNPs (-137 G>C, "rs187238"; -607 C>A, "rs1946518"; +105A>C, "rs549908"; and -656 G>T, "rs1946519") and RSA in the study population. Future work should focus on association of other IL-18 and immune response genes' polymorphisms in RSA cases.
|Publisher||الجامعة الإسلامية - غزة|
|Files in this item|