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|Title||Hepcidin Status Correlated with Biochemical Parameters among Iron Deficient Anemic Children aged (6–12) Years in Gaza City|
Background: Hepcidin is a small cysteine-rich peptide hormone produced in the liver. Hepcidin is also a tightly folded polypeptide containing 25 residues in length and is 32% beta sheets. This molecule regulates the absorption of iron in the body. It was discovered in 2000. Recent studies demonstrated that hepcidin is a master iron regulator. Therefore, assessment of hepcidin status and clarifying its association in iron deficiency anemia (IDA) could constitute a promising therapy of the disease. Objective: To correlate hepcidin status with some biochemical parameters among IDA children aged 6 – 12 years inGazaCity. Materials and Methods: This case-control study comprised 80 IDA children and 80 apparently healthy non IDA children controls. Questionnaire interviews were applied. Serum hepcidin and serum ferritin were measured by ELISA. Serum iron, and TIBC were determined photometrically. Complete blood count (CBC) was also performed by [Cell-Dyn-1800] autoanalyser. Transferrin and transferrin saturation were calculated. We obtained a permission from local ethical committee to conduct this study. Overall data were computer analyzed using SPSS package version 20.0. Results: The mean serum iron, transferrin saturation and serum ferritin in cases were significantly lower than that in controls ( 48.4±16.3 μg/dL, 13.8±6.3 % and 13.4±7.2 ng/ml vs. 80.7±21.3 μg/dL, 25.9±7.4 % and 20.6±12.7 ng/ml) with P=0.000. Hepcidin was found to be significantly associated with family income and children physical activity. The mean level of serum hepcidin was significantly lower in IDA children compared to healthy non IDA children controls (4.8±5.7 and 8.0±6.7 ng/ml, P=0.001). The Pearson correlation test showed negative significant correlations between hepcidin levels and serum iron (r=-0.232, P= 0.003), and positive significant correlations with serum ferritin (r=0.320 P=0.000). The mean TIBC and transferrin in cases were significantly higher than that in controls (367.9±41.4 μg/dL and 262.1±29.5 mg/dL vs 315.6±38.9 μg/dL and 224.9±27.7 mg/dL) with P=0.000. The Pearson correlation test showed negative significant correlations between hepcidin levels and TIBC and transferrin (r=-0.172, P= 0.030, r=-0.168, P=0.033, respectively). The average values of RBC, Hb, HCT, MCV, MCH and MCHC were significantly lower in IDA children (4.6±0.7, 10.3±0.5 g/dl, 32.2±2.5, 71.1±8.8, 22.9±3.4 and 32.0±1.7, respectively). Compared to controls (4.8±0.4, 12.0±0.7 g/dl, 35.8±2.1, 75.6±4.7, 25.7±2.0 and 33.4±1.6, respectively with P= 0.000). On the other hand, RDW was significantly higher in cases vs controls (14.8±2.6 vs 13.7±0.8, with P=0.000). Pearson correlation test also showed positive significant correlations between hepcidin levels and RBC and Hb.Conclusions: The mean level of hepcidin was significantly lower in cases compared to controls. Hepcidin levels were significantly higher among children whose parents were more educated and employed, as well as high income families. IDA was more prevalent among children whose parents have a lower education level and unemployed, as well as in families with less income and individuals with family history of IDA. Hepcidin is strongly correlated with serum iron, transferrin saturation and serum ferritin. Thus it is considered as a good marker and promising therapeutic agent of IDA. Thus; It is recommended to introduce hepcidin hormone assay for IDA in our area, and conduct further research related to the relationship of hepcidin hormone with IDA.
|Publisher||الجامعة الإسلامية - غزة|
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