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|Title||Isolation and Characterization of Methamidophos Biodegrading Microorganisms from Soil|
Pesticides are important production inputs for many agricultural supplies, and decisions concerning their use require balancing factors between their economic benefits and the protection of the environment and human health. Microorganisms play key roles in the biodegradation of pesticide-contaminated sites. Methamidophos is one of the most widely used organophosphorus insecticides usually detectable in the environment. Methamidophos is considered as one of the most toxic insecticides; it can cause acute toxic effects by the inhibition of acetylcholinesterase enzyme activity in nervous tissue and can significantly damage peoples’ health. Methamidophos was often detected in high concentrations in the ecosystem and thus lead to some direct and potential adverse effects on environmental safety. However, few studies of the microbial degradation of methamidophos have been carried out. For this project, we proposed a model for methamidophos biodegradation by bacteria grown in soil samples sites in agricultural area which had a history of methamidophos exposure. The pesticides Methamidophos was used in a minimal salt medium as a sole source of carbon inorder to isolate and characterize the methamidophos degraders. Strains of Neisseria lactamica, Micrococcus lylae and Bacillus sphaericus were isolated from Methamidophos contaminated soil and identified as mehamidophos degraders and were found to use methamidophos as a sole carbon source. The presented data offers useful information about the biodegradation mechanism of methamidophos using microorganisms and can be used in the bidegradation of pesticide-contaminated sites.
|Publisher||the islamic university|
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