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|Title||Activity Behavior of Human Serum Albumin Doped in Silica Sol-Gel Thin Film|
|Title in Arabic||دراسة نشاط سلوك الالبومين البشري المندمج في افلام رقيقة من السليكا باستخدام طريقة الصل جل|
Transparent sol-gel thin films immobilized with human serum albumin (HSA) were made via the acid catalyzed sol-gel reaction of tetraethoxysilane (TEOS) in presence of the human serum albumin. Different surfactants that include cationic cetyl trimethyl ammonium bromide (CTAB), anionic sodium dodecyl sulfate (SDS) and nonionic Triton X-100 (TX-100) were tested for the improvement of the host material mesostructure , increasing its porosity and well accommodation of the HSA protein within the silica matrix. The thin films show similar behavior in presence of SDS surfactant as their free counterparts in aqueous solution with a significant shift in the wavelength (~ 10 nm) of UV absorption spectra. The immobilized human serum albumin retained its activity by immobilization. Different parameters include concentration of protein and surfactant, type of surfactant, life time and number of measurements were investigated. The HSA thin film sensor showed stability, repeatability, reproducibility and long life time behavior. Maximum stability of HSA thin films were achieved by drying at 37oC.
|Publisher||الجامعة الإسلامية - غزة|
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