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http://hdl.handle.net/20.500.12358/24385
TitleNormal-phase liquid chromatographic separation of stratum corneum ceramides with detection by evaporative light scattering and atmospheric pressure chemical ionization mass …
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Abstract

A new normal-phase HPLC method for the separation of stratum corneum ceramides is presented. Separation was achieved in less than 15 min using a binary gradient consisting of chloroform, n-propanol and acetic acid. Since UV and fluorescence detection are not possible without derivatization and electrospray MS is not compatible to normal-phase LC, we have investigated evaporative light scattering detection (ELSD) and atmospheric pressure chemical ionization mass spectrometry (APCI-MS). When comparing both detection methods, it can be stated that APCI-MS was more sensitive and provides more structural information. However, ELSD can be an economic alternative for routine analysis. The method was applied to human stratum corneum ceramides obtained by lipid extraction. It was shown that the peaks found in LC correspond to the bands described for TLC. The conversion of TLC procedures to …

Authors
Farwanah, Hany
Nuhn, Peter
Neubert, Reinhard HH
Raith, Klaus
TypeJournal Article
Date2003
Published inAnalytica chimica acta
SeriesVolume: 492, Number: 1
PublisherElsevier
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  • Staff Publications- Faculty of Medicine [149]
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The institutional repository of the Islamic University of Gaza was established as part of the ROMOR project that has been co-funded with support from the European Commission under the ERASMUS + European programme. This publication reflects the views only of the author, and the Commission cannot be held responsible for any use which may be made of the information contained therein.

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The institutional repository of the Islamic University of Gaza was established as part of the ROMOR project that has been co-funded with support from the European Commission under the ERASMUS + European programme. This publication reflects the views only of the author, and the Commission cannot be held responsible for any use which may be made of the information contained therein.

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