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http://hdl.handle.net/20.500.12358/24380
TitleImproved procedure for the separation of major stratum corneum lipids by means of automated multiple development thin-layer chromatography
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Abstract

The separation of the major stratum corneum lipids, i.e., ceramides, fatty acids, cholesterol and its esters by means of high-performance thin-layer chromatography is hereby presented. The used automated multiple development technique allows the reproducible development of a 17-step solvent gradient also capable of separating seven ceramide classes in the same run. Reliable quantification has been performed after visualisation and densitometric scanning. The present approach is less time and solvent-consuming than previously described procedures. The application to samples obtained by in vivo skin surface extraction with hexane–ethanol (2:1) demonstrates that the method can be routinely used for diagnostic purposes.

Authors
Farwanah, Hany
Neubert, Reinhard HH
Zellmer, Sebastian
Raith, Klaus
TypeJournal Article
Date2002
Published inJournal of Chromatography B
SeriesVolume: 780, Number: 2
PublisherElsevier
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  • Staff Publications- Faculty of Medicine [149]
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The institutional repository of the Islamic University of Gaza was established as part of the ROMOR project that has been co-funded with support from the European Commission under the ERASMUS + European programme. This publication reflects the views only of the author, and the Commission cannot be held responsible for any use which may be made of the information contained therein.

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The institutional repository of the Islamic University of Gaza was established as part of the ROMOR project that has been co-funded with support from the European Commission under the ERASMUS + European programme. This publication reflects the views only of the author, and the Commission cannot be held responsible for any use which may be made of the information contained therein.

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