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Normal phase liquid chromatography coupled to quadrupole time of flight atmospheric pressure chemical ionization mass spectrometry for separation, detection and mass …

Farwanah, Hany; Wirtz, Jennifer; Kolter, Thomas; Raith, Klaus; Neubert, Reinhard HH; Sandhoff, Konrad (Elsevier, 2009)

Many lipidomic approaches focus on investigating aspects of sphingolipid metabolism. Special emphasis is put on neutral sphingolipids and cholesterol and their interaction. Such an interest is attributed to the fact that ...

Separation and mass spectrometric characterization of covalently bound skin ceramides using LC/APCI-MS and Nano-ESI-MS/MS

Farwanah, Hany; Pierstorff, Barbara; Schmelzer, Christian EH; Raith, Klaus; Neubert, Reinhard HH; Kolter, Thomas; Sandhoff, Konrad (Elsevier, 2007)

Ceramides covalently bound to keratinocytes are essential for the barrier function of the skin, which can be disturbed in diseases, such as psoriasis and atopic dermatitis. These ceramides of the classes ω-hydroxyacyl-sphingosine ...

Induction of a hardening phenomenon by repeated application of SLS: analysis of lipid changes in the stratum corneum

Heinemann, Christian; Paschold, Christiane; Fluhr, Joachim; Wigger-Alberti, Walter; Schliemann-Willers, Sibylle; Farwanah, Hany; Raith, Klaus; Neubert, Reinhard HH; Elsner, Peter (2005)

Adaptation of the skin to repeated influence of exogenous irritants is called the hardening phenomenon. We investigated the stratum corneum lipid composition before and after induction of a hardening phenomenon. Irritant ...

A new LC/APCI-MS method for the determination of cholesterol oxidation products in food

Raith, Klaus; Brenner, Christian; Farwanah, Hany; Müller, Gerit; Eder, Klaus; Neubert, Reinhard HH (Elsevier, 2005)

Cholesterol oxidation products (COPs) can be formed in the body or in animal foods from cholesterol during food processing. A new method for the extraction and quantification of cholesterol, 7-ketocholesterol, cholestane ...

Ceramide profiles of the uninvolved skin in atopic dermatitis and psoriasis are comparable to those of healthy skin

Farwanah, Hany; Raith, Klaus; Neubert, Reinhard HH; Wohlrab, Johannes (Springer-Verlag, 2005)

Ceramides are sphingolipids consisting of sphingoidbases, which are amide-linked to fatty acids. In the stratum corneum, they represent the major constituent of the free extractable intercellular lipids and play a significant ...

Profiling of human stratum corneum ceramides by means of normal phase LC/APCI–MS

Farwanah, Hany; Wohlrab, Johannes; Neubert, Reinhard HH; Raith, Klaus (Springer-Verlag, 2005)

The ceramides of the stratum corneum are critical to maintaining the epidermal barrier function of the skin. A number of skin diseases and disorders are known to be related to impairments of the ceramide pattern. Therefore, ...

Progress in the analysis of stratum corneum ceramides

Raith, Klaus; Farwanah, Hany; Wartewig, Siegfried; Neubert, Reinhard HH (WILEY‐VCH Verlag, 2004)

The stratum corneum with its unique structure of corneocytes and intercellular lipid lamellae enables a protection of human and other mammalian skin against transepidermal water loss and harmful substances from the ...

Normal-phase liquid chromatographic separation of stratum corneum ceramides with detection by evaporative light scattering and atmospheric pressure chemical ionization mass …

Farwanah, Hany; Nuhn, Peter; Neubert, Reinhard HH; Raith, Klaus (Elsevier, 2003)

A new normal-phase HPLC method for the separation of stratum corneum ceramides is presented. Separation was achieved in less than 15 min using a binary gradient consisting of chloroform, n-propanol and acetic acid. Since ...

Improved procedure for the separation of major stratum corneum lipids by means of automated multiple development thin-layer chromatography

Farwanah, Hany; Neubert, Reinhard HH; Zellmer, Sebastian; Raith, Klaus (Elsevier, 2002)

The separation of the major stratum corneum lipids, i.e., ceramides, fatty acids, cholesterol and its esters by means of high-performance thin-layer chromatography is hereby presented. The used automated multiple development ...

LC/APCI-MS in Lipid Analysis

Raith, Klaus; Farwanah, Hany; Brenner, Christian; Schmelzer, Christian EH; Neubert, Reinhard HH

Atmospheric Pressure Chemical Ionisation (APCI) is a well-established ionisation technique for liquid samples. Although LC/MS is mostly carried out using the electrospray interface, APCI and the relatively new Atmospheric ...