Browsing by Author "hfarwanah"
Now showing items 1-20 of 21
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A new LC/APCI-MS method for the determination of cholesterol oxidation products in food
(Elsevier, 2005)Cholesterol oxidation products (COPs) can be formed in the body or in animal foods from cholesterol during food processing. A new method for the extraction and quantification of cholesterol, 7-ketocholesterol, cholestane ... -
Ablation of neuronal ceramide synthase 1 in mice decreases ganglioside levels and expression of myelin-associated glycoprotein in oligodendrocytes
(American Society for Biochemistry and Molecular Biology, 2012)Background: Ceramide synthase 1 catalyzes the synthesis of C18 ceramide and is mainly expressed in neurons of the brain. Results: Ablation of Ceramide Synthase 1 decreases ganglioside levels and expression of oligodendrocytic ... -
Activation of Nrf2 in keratinocytes causes chloracne (MADISH)‐like skin disease in mice
(EMBO Press, 2014)The transcription factor Nrf2 is a key regulator of the cellular stress response, and pharmacological Nrf2 activation is a promising strategy for skin protection and cancer prevention. We show here that prolonged Nrf2 ... -
Adult ceramide synthase 2 (CERS2)-deficient mice exhibit myelin sheath defects, cerebellar degeneration, and hepatocarcinomas
(American Society for Biochemistry and Molecular Biology, 2009)(Dihydro) ceramide synthase 2 (cers2, formerly called lass2) is the most abundantly expressed member of the ceramide synthase gene family, which includes six isoforms in mice. CERS2 activity has been reported to be specific ... -
Ceramide profiles of the uninvolved skin in atopic dermatitis and psoriasis are comparable to those of healthy skin
(Springer-Verlag, 2005)Ceramides are sphingolipids consisting of sphingoidbases, which are amide-linked to fatty acids. In the stratum corneum, they represent the major constituent of the free extractable intercellular lipids and play a significant ... -
CLN3 loss disturbs membrane microdomain properties and protein transport in brain endothelial cells
(Society for Neuroscience, 2013)Juvenile neuronal ceroid lipofuscinosis (JNCL) is a fatal childhood-onset neurodegenerative disorder caused by mutations in ceroid lipofuscinosis neuronal-3 (CLN3), a hydrophobic transmembrane protein of unresolved function. ... -
Improved procedure for the separation of major stratum corneum lipids by means of automated multiple development thin-layer chromatography
(Elsevier, 2002)The separation of the major stratum corneum lipids, i.e., ceramides, fatty acids, cholesterol and its esters by means of high-performance thin-layer chromatography is hereby presented. The used automated multiple development ... -
Induction of a hardening phenomenon by repeated application of SLS: analysis of lipid changes in the stratum corneum
(Acta Dermato-Venereologica, 2005)Adaptation of the skin to repeated influence of exogenous irritants is called the hardening phenomenon. We investigated the stratum corneum lipid composition before and after induction of a hardening phenomenon. Irritant ... -
LC/APCI-MS in Lipid Analysis
Atmospheric Pressure Chemical Ionisation (APCI) is a well-established ionisation technique for liquid samples. Although LC/MS is mostly carried out using the electrospray interface, APCI and the relatively new Atmospheric ... -
Lipidomics
(John Wiley & Sons, Inc., 2007)Lipids are hydrophobic or amphiphilic low‐molecular‐weight substances with a low solubility in water. Glycerophospholipids, sphingolipids, and cholesterol are the building blocks of cellular membranes, and triacylglycerols ... -
Lipidomics of glycosphingolipids
(Molecular Diversity Preservation International, 2012): Glycosphingolipids (GSLs) contain one or more sugars that are attached to a sphingolipid moiety, usually to a ceramide, but in rare cases also to a sphingoid base. A large structural heterogeneity results from differences ... -
Loss of CB1 receptors leads to decreased cathepsin D levels and accelerated lipofuscin accumulation in the hippocampus
(Elsevier, 2013)Early onset of age-related changes in the brain of cannabinoid 1 receptor knockout (Cnr1−/−) mice suggests that cannabinoid 1 (CB1) receptor activity significantly influences the progression of brain aging. In the present ... -
Mass spectrometric analysis of neutral sphingolipids: methods, applications, and limitations
(Elsevier, 2011)Sphingolipids represent an important class among lipids, especially when considering their vital roles in lipid metabolism. Thus, a variety of methods have been created to accomplish their analysis and the term “sphingolipidomics” ... -
Normal phase liquid chromatography coupled to quadrupole time of flight atmospheric pressure chemical ionization mass spectrometry for separation, detection and mass …
(Elsevier, 2009)Many lipidomic approaches focus on investigating aspects of sphingolipid metabolism. Special emphasis is put on neutral sphingolipids and cholesterol and their interaction. Such an interest is attributed to the fact that ... -
Normal-phase liquid chromatographic separation of stratum corneum ceramides with detection by evaporative light scattering and atmospheric pressure chemical ionization mass …
(Elsevier, 2003)A new normal-phase HPLC method for the separation of stratum corneum ceramides is presented. Separation was achieved in less than 15 min using a binary gradient consisting of chloroform, n-propanol and acetic acid. Since ... -
Nrf2 links epidermal barrier function with antioxidant defense
(EMBO Press, 2012)The skin provides an efficient permeability barrier and protects from microbial invasion and oxidative stress. Here, we show that these essential functions are linked through the Nrf2 transcription factor. To test the ... -
Profiling of human stratum corneum ceramides by means of normal phase LC/APCI–MS
(Springer-Verlag, 2005)The ceramides of the stratum corneum are critical to maintaining the epidermal barrier function of the skin. A number of skin diseases and disorders are known to be related to impairments of the ceramide pattern. Therefore, ... -
Progress in the analysis of stratum corneum ceramides
(WILEY‐VCH Verlag, 2004)The stratum corneum with its unique structure of corneocytes and intercellular lipid lamellae enables a protection of human and other mammalian skin against transepidermal water loss and harmful substances from the ... -
Schlank, a member of the ceramide synthase family controls growth and body fat in Drosophila
(EMBO Press, 2009)Ceramide synthases are highly conserved transmembrane proteins involved in the biosynthesis of sphingolipids, which are essential structural components of eukaryotic membranes and can act as second messengers regulating ... -
Separation and mass spectrometric characterization of covalently bound skin ceramides using LC/APCI-MS and Nano-ESI-MS/MS
(Elsevier, 2007)Ceramides covalently bound to keratinocytes are essential for the barrier function of the skin, which can be disturbed in diseases, such as psoriasis and atopic dermatitis. These ceramides of the classes ω-hydroxyacyl-sphingosine ...